V3N307 Observation on the stability of rat myelosuppression model established by different doses of cyclophosphamide solution

By | 02/11/2021

TAN Li1,XIE Yu2,CHEN Hongda3,WANG Ning3

1Shenzhen Longhua District Central Hospital, Shenzhen 518110, China

2Co-first author: XIE Yu, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510006, China

3The seventh Affiliated Hospital,Sun Yat sen University, Shenzhen,518107, China

Corresponding author: WANG Ning, wangn79@mail.sysu.edu.cn,https://orcid.org/0000-0002-0972-2486

Co corresponding author: CHEN Hongda,41370711@qq.com

Supported by National Natural Science Fund of China (Youth Program No. 81804171) 

MRHK20210307

Article History Received 5 June 2021  Accepted 25 July 2021  Published 30 September 2021

Cite this Article TAN Li, XIE Yu, CHEN Hongda, WANG Ning. Observation on the stability of rat myelosuppression model established by different doses of cyclophosphamide solution [J].Medical Research, 2021.3(3):65-72, http://dx.doi.org/mrhk/10.6913/MRHK.202109_3(3).0007

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Abstract  Objective to investigate the stability of different doses of cyclophosphamide solution in establishing rat myelosuppression model. Methods In this study, 30 SD rats were randomly divided into control group (Group C) and model group (M1, M2, M3, M4). The rats in the model groups were injected with cyclophosphamide solution by intraperitoneal injection at doses of 30、40、(30+15) and (40+20) mg/kg respectively to establish a bone marrow suppression rat model. The changes of WBC, lym, Mon, GRA, RBC, Hb and PLT in peripheral blood were observed on the 1st, 5th and 13th day after modeling. Results (1)After modeling, the weight of rats in the model group was lower than that in the control group (P < 0.05). (2)On the 5th day, the levels of WBC, lym, mon and gra in the serum of each model group were lower than those before modeling(P < 0.05). The content of RBC in M1 and M2 groups was lower than that before modeling (P < 0.05), the content of Hb in M1, M2 and M3 groups was lower than that before modeling (P < 0.05), and the content of PLT in M1, M2, m3 and M4 groups was lower than that before modeling (P < 0.05). (3)On the 13th day, the levels of WBC, lym, mon and gra in M1 and M2 groups were higher than those on the 5th day, except for the GRA in M2 group.The levels of WBC and lym in m3 and M4 were lower than those in M1 and M2 (P < 0.05); The contents of RBC and Hb in M1 and M2 groups were lower than those before modeling (P < 0.05); Compared with M1 and M2, the content of Hb in m3 and M4 groups was higher (P < 0.05); The PLT content in the peripheral blood of M3 and M4 rats was higher than that of the 5th day, but lower than that of M1 and M2 groups (P < 0.05). Conclusion The improved method can maintain the stability of rat myelosuppression model.

Key words Cyclophosphamide;rat; myelosuppression model