Yu Fei1*,Tan Yuan-fei1*, Sheng Guan-nan2, Chen Guan-nan2, Wang Nan-nan2, Gong Ting2, Qu Bo3#
1.Emergency Department, the Seventh Affiliated Hospital, Sun Yat-sen University, Shen Zhen,518107;Yu Fei, Email E-mail: doctor_yuf@126.com, https://orcid.org/0000-0001-5312-3328; Tan Yuan-fei, Email tyf.0604@163.com, https://orcid.org/0000-0001-5985-6952 2.Jinzhou Medical University, Jin Zhou, 121002
2.Jinzhou Medical University, Jin Zhou,121002
3.Institute of Disaster Medicine, Tianjin University, Tian Jin, 300162
*Yu Fei andTan Yuan-fei are co-first authors. #Qu Bo is the corresponding author.
Received:1 December 2019 Accepted: 5 December 2019 Published: 31 December 2019
Cite this Article Yu Fei, Tan Yuan-fei , Sheng Guan-nan, Chen Guan-nan, Wang Nan-nan, Gong Ting, Qu Bo. The construction of a sheep acute respiratory distress syndrome model induced by smoke inhalation injury [J].Medical Research,2019.1(1): 1-11, Http://dx.doi.org/10.6913/MRHK.201912_1(1).0001
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ABSTRACT
Objective To establish a model of acute respiratory distress syndrome induced by sheep smoke inhalation lung injury.
Methods Nine healthy, 12-24 month old, non-pregnant female sheep were randomly selected, the sheep were divided into three groups, through the internal control of smoke generator device components (CO, CO2, O2, particle concentration and smoke temperature, etc.) to adjust the exposure time of the sheep model construction of smoke. The chest CT were examined before and after exposure to smoke at 6h, 12h, 24h and 48h, respectively. From the smoke exposure 12h endotracheal intubation and mechanical ventilation, by adjusting the ventilator parameters of hypoxemia and hypercapnia, statistical analysis of PaO2/FiO2, PEEP and other indicators, according to the ARDS definition of diagnosis in Berlin definition.
Results The CT showed mild inflammation of the lungs in first group, oxygen index of 95% confidence interval [331.13, 353.53]; In second groups, oxygenation index 95% confidence interval [220.09, 383.90]; CT showed airway edema, pulmonary inflammatory exudation and lung injury in third group. Oxygenation index 95% confidence interval of [195.16, 244.84], PEEP>5cm. There was significant difference between the two groups (P<0.05).
Conclusions The severity of lung injury can be changed by adjusting the exposure time of smoke inhalation; when the smoke exposure time up to 45 minutes, a stable model of smoke inhalation lung injury in sheep ARDS can be successfully established.
Key words：Sheep; smoke inhalation injury; acute respiratory distress syndrome; model; mechanical ventilation